Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0
Identifieur interne : 005367 ( Main/Exploration ); précédent : 005366; suivant : 005368Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0
Auteurs : Leonie-Sophie Hecht [Allemagne] ; Angeles Jurado-Jimenez [Allemagne] ; Markus Hess [Allemagne] ; Hussein El Halas [Allemagne] ; Gregor Bochenek [Allemagne] ; Hala Mohammed [Arabie saoudite] ; Fahd Alzahrani [Arabie saoudite] ; Mohammed O. Asiri [Arabie saoudite] ; Rami Hasan [Arabie saoudite] ; Aref Alamri [Arabie saoudite] ; Sultan Alotaibi [Arabie saoudite]Source :
- Future Microbiology [ 1746-0913 ] ; ????.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Arabie saoudite, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Protéines nucléocapside (génétique), Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines nucléocapside.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- Arabie saoudite, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Nucleocapsid Proteins (genetics), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction, Saudi Arabia, Sensitivity and Specificity.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, RNA, Viral.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques.
- virology : Coronavirus Infections.
- Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene.
33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV
18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results.
The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.
Url:
DOI: 10.2217/fmb-2019-0067
PubMed: 31271059
PubMed Central: 7079715
Affiliations:
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Le document en format XML
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nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Halas, Hussein El" sort="Halas, Hussein El" uniqKey="Halas H" first="Hussein El" last="Halas">Hussein El Halas</name><affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Bochenek, Gregor" sort="Bochenek, Gregor" uniqKey="Bochenek G" first="Gregor" last="Bochenek">Gregor Bochenek</name><affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Mohammed, Hala" sort="Mohammed, Hala" uniqKey="Mohammed H" first="Hala" last="Mohammed">Hala Mohammed</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alzahrani, Fahd" sort="Alzahrani, Fahd" uniqKey="Alzahrani F" first="Fahd" last="Alzahrani">Fahd Alzahrani</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Asiri, Mohammed O" sort="Asiri, Mohammed O" uniqKey="Asiri M" first="Mohammed O" last="Asiri">Mohammed O. 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xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alotaibi, Sultan" sort="Alotaibi, Sultan" uniqKey="Alotaibi S" first="Sultan" last="Alotaibi">Sultan Alotaibi</name><affiliation wicri:level="1"><nlm:aff id="AFF3">King Fahad Medical City, Riyadh, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author></analytic><series><title level="j">Future Microbiology</title><idno type="ISSN">1746-0913</idno><idno type="eISSN">1746-0921</idno><imprint><date when="????">????</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Nucleocapsid Proteins (genetics)</term><term>RNA, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction</term><term>Saudi Arabia</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Arabie saoudite</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Protéines nucléocapside (génétique)</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arabie saoudite</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Aim:</title><p>We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene.</p></sec><sec><title>Material & methods:</title><p>33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV <italic>orf1a</italic> and <italic>upE</italic>, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO.</p></sec><sec><title>Results:</title><p>18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results.</p></sec><sec><title>Conclusion:</title><p>The results suggest, that the RealStar<sup>®</sup> MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ogden, Nh" uniqKey="Ogden N">NH Ogden</name></author><author><name sortKey="Abdelmalik, P" uniqKey="Abdelmalik P">P Abdelmalik</name></author><author><name sortKey="Pulliam, Jrc" uniqKey="Pulliam J">JRC Pulliam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM 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Huang</name></author><author><name sortKey="Lin, P H" uniqKey="Lin P">P-H Lin</name></author><author><name sortKey="Tsai, K W" uniqKey="Tsai K">K-W Tsai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Olschl Ger, S" uniqKey="Olschl Ger S">S Ölschläger</name></author><author><name sortKey="Wendtner, C M" uniqKey="Wendtner C">C-M Wendtner</name></author><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Hess, M" uniqKey="Hess M">M Hess</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>Arabie saoudite</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><country name="Allemagne"><region name="Hambourg"><name sortKey="Hecht, Leonie Sophie" sort="Hecht, Leonie Sophie" uniqKey="Hecht L" first="Leonie-Sophie" last="Hecht">Leonie-Sophie Hecht</name></region><name sortKey="Bochenek, Gregor" sort="Bochenek, Gregor" uniqKey="Bochenek G" first="Gregor" last="Bochenek">Gregor Bochenek</name><name sortKey="Halas, Hussein El" sort="Halas, Hussein El" uniqKey="Halas H" first="Hussein El" last="Halas">Hussein El Halas</name><name sortKey="Hess, Markus" sort="Hess, Markus" uniqKey="Hess M" first="Markus" last="Hess">Markus Hess</name><name sortKey="Jurado Jimenez, Angeles" sort="Jurado Jimenez, Angeles" uniqKey="Jurado Jimenez A" first="Angeles" last="Jurado-Jimenez">Angeles Jurado-Jimenez</name></country><country name="Arabie saoudite"><noRegion><name sortKey="Mohammed, Hala" sort="Mohammed, Hala" uniqKey="Mohammed H" first="Hala" last="Mohammed">Hala Mohammed</name></noRegion><name sortKey="Alamri, Aref" sort="Alamri, Aref" uniqKey="Alamri A" first="Aref" last="Alamri">Aref Alamri</name><name sortKey="Alotaibi, Sultan" sort="Alotaibi, Sultan" uniqKey="Alotaibi S" first="Sultan" last="Alotaibi">Sultan Alotaibi</name><name sortKey="Alzahrani, Fahd" sort="Alzahrani, Fahd" uniqKey="Alzahrani F" first="Fahd" last="Alzahrani">Fahd Alzahrani</name><name sortKey="Asiri, Mohammed O" sort="Asiri, Mohammed O" uniqKey="Asiri M" first="Mohammed O" last="Asiri">Mohammed O. Asiri</name><name sortKey="Hasan, Rami" sort="Hasan, Rami" uniqKey="Hasan R" first="Rami" last="Hasan">Rami Hasan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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